Coelho lab

• Base editing screens define the genetic landscape of cancer drug resistance mechanisms Matthew A. Coelho^*, Magdalena E. Strauss, Alex Watterson, Sarah Cooper, Shriram Bhosle, Giuditta Illuzzi, Emre Karakoc, Cansu Dinçer, Sara F. Vieira, Mamta Sharma, Marie Moullet, Daniela Conticelli, Jonas Koeppel, Katrina McCarten, Chiara M. Cattaneo, Vivien Veninga, Gabriele Picco, Leopold Parts, Josep V. Forment, Emile E. Voest, John C. Marioni, Andrew Bassett & Mathew J. Garnett^* 2024 Nature Genetics

• Base editing screens map mutations affecting interferon-γ signaling in cancer Matthew A Coelho, Sarah Cooper, Magdalena E Strauss, Emre Karakoc, Shriram Bhosle, Emanuel Goncalves, Gabriele Picco, Thomas Burgold, Chiara M Cattaneo, Vivien Veninga, Sarah Consonni, Cansu Dincer, Sara F Vieira, Freddy Gibson, Syd Barthorpe, Claire Hardy, Joel Rein, Mark Thomas, John Marioni, Emile E Voest, Andrew Bassett, Mathew J Garnett 2023/2/13 Cancer Cell

• scSNV-seq: high-throughput phenotyping of single nucleotide variants by coupled single-cell genotyping and transcriptomics Sarah E Cooper*, Matthew A Coelho*, Magdalena E Strauss*, Aleksander M Gontarczyk, Qianxin Wu, Mathew J Garnett, John C Marioni, Andrew R Bassett 2024/1/15 Genome Biology

• Novel WRN Helicase Inhibitors Selectively Target Microsatellite-Unstable Cancer Cells Gabriele Picco, Yanhua Rao, Angham Al Saedi, Yang Lee, Sara F Vieira, Shriram Bhosle, Kieron May, Carmen Herranz-Ors, Samantha J Walker, Raynold Shenje, Cansu Dincer, Freddy Gibson, Ruby Banerjee, Zoe Hewitson, Thilo Werner, Joshua E Cottom, Yang Peng, Nanhua Deng, Youyou Zhang, Eldridge N Nartey, Leng Nickels, Philip Landis, Daniela Conticelli, Katrina McCarten, Jacob Bush, Mamta Sharma, Howard Lightfoot, David House, Emma Milford, Emma K Grant, Michal P Glogowski, Craig D Wagner, Marcus Bantscheff, Anna Rutkowska-Klute, Cell Model Network UK Group, Francesca Zappacosta, Jonathan Pettinger, Syd Barthorpe, H Christian Eberl, Brian T Jones, Jessica L Schneck, Dennis J Murphy, Emile E Voest, Joshua P Taygerly, Michael P DeMartino, Matthew A Coelho, Jonathan Houseley, Geeta Sharma, Benjamin Schwartz, Mathew J Garnett 2024/8/2 Cancer Discovery

• Analyzing the functional effects of DNA variants with gene editing Sarah Cooper, Sofia Obolenski, Andrew J Waters, Andrew R Bassett, Matthew A Coelho 2024/5/13 Cell Reports Methods

Gene editing technologies

• High-content CRISPR screening Christoph Bock, Paul Datlinger, Florence Chardon, Matthew A Coelho, Matthew B Dong, Keith A Lawson, Tian Lu, Laetitia Maroc, Thomas M Norman, Bicna Song, Geoff Stanley, Sidi Chen, Mathew Garnett, Wei Li, Jason Moffat, Lei S Qi, Rebecca S Shapiro, Jay Shendure, Jonathan S Weissman, Xiaowei Zhuang 2022/2/10 Nature Reviews Methods Primers

• CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs Matthew A Coelho*, Etienne De Braekeleer, Mike Firth, Michal Bista, Sebastian Lukasiak, Maria Emanuela Cuomo, Benjamin JM Taylor* 2020/8/17 Nature communications

• BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B Matthew A Coelho*, Songyuan Li, Luna Simona Pane, Mike Firth, Giovanni Ciotta, Jonathan D Wrigley, Maria Emanuela Cuomo, Marcello Maresca, Benjamin JM Taylor* 2018/12 BMC biology

• Universal toxin-based selection for precise genome engineering in human cells Songyuan Li, Nina Akrap, Silvia Cerboni, Michelle J Porritt, Sandra Wimberger, Anders Lundin, Carl Möller, Mike Firth, Euan Gordon, Bojana Lazovic, Aleksandra Sieńska, Luna Simona Pane, Matthew A Coelho, Giovanni Ciotta, Giovanni Pellegrini, Marcella Sini, Xiufeng Xu, Suman Mitra, Mohammad Bohlooly-Y, Benjamin JM Taylor, Grzegorz Sienski, Marcello Maresca 2021/1/21 Nature communications

Lung cancer and immunotherapy

• Oncogenic RAS signaling promotes tumor immunoresistance by stabilizing PD-L1 mRNA Matthew A Coelho, Sophie de Carné Trécesson, Sareena Rana, Davide Zecchin, Christopher Moore, Miriam Molina-Arcas, Philip East, Bradley Spencer-Dene, Emma Nye, Karin Barnouin, Ambrosius P Snijders, Wi S Lai, Perry J Blackshear, Julian Downward 2017/12/19 Immunity

• An immunogenic model of KRAS-mutant lung cancer enables evaluation of targeted therapy and immunotherapy combinations Jesse Boumelha, Sophie de Carné Trécesson, Emily K Law, Pablo Romero-Clavijo, Matthew A Coelho, Kevin W Ng, Edurne Mugarza, Christopher Moore, Sareena Rana, Deborah R Caswell, Miguel Murillo, David C Hancock, Prokopios P Argyris, William L Brown, Cameron Durfee, Lindsay K Larson, Rachel I Vogel, Alejandro Suarez-Bonnet, Simon L Priestnall, Philip East, Sarah J Ross, George Kassiotis, Miriam Molina-Arcas, Charles Swanton, Reuben Harris, Julian Downward 2022/10/4 Cancer research

• Cancer immune evasion through KRAS and PD-L1 and potential therapeutic interventions Alex Watterson, Matthew A Coelho 2023/3/2 Cell Communication and Signaling

• Breaking barriers: we need a multidisciplinary approach to tackle cancer drug resistance James Ingham, Jia-Ling Ruan & Matthew A Coelho 2024/3/1 BJC Reports